Among fungal isolates from seeds of Diospyros crassiflora, one showed cultural and microscopic features of Pestalotiopsis species. DNA-sequence comparison and phylogenetic analyses using nucleotide sequences of internal transcribed spacer (ITS1-5.8S-ITS2) and the portion of nuclear large subunit (nuc-LSU) rDNA identified it as Pestalotiopsis theae. This finding indicated that Pestalotiopsis theae, a common pathogen that is often an endophyte or saprobc, may also be seminicolous.